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quite imposing plus 3.0 serial number, quite imposing plus 3.0 keygen, quite imposing plus 3.0 serial number, quite imposing plus 3.0 crack download, quite imposing plus 3.0 product key, quite imposing plus 3.0 serial .\]. The current study can serve as a model example for the use of phenotypic analysis on multiple media.
Biofilms are dynamic systems in which gene expression and behaviour change according to environmental conditions \[[@CR23]\]. The ability to culture *P. aeruginosa* biofilms in vitro could be a significant help in understanding the mechanisms of antimicrobial resistance in *P. aeruginosa* biofilms. It is particularly important to characterize the transcriptome of *P. aeruginosa* biofilms to identify the genes involved in causing this increased antimicrobial resistance, and the mechanisms involved.
Conclusion {#Sec12}
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In this study, we demonstrated that we can successfully culture *P. aeruginosa* biofilms on QCM chips, and that we can use these biofilms to track the effect of media on antimicrobial activity over time. Therefore, we can use QCM chips as a method to observe the effect of media on *P. aeruginosa* biofilms, which can aid in the development of a better understanding of antimicrobial resistance during biofilm formation.
Methods {#Sec13}
=======
Bacterial strains, media, and growth conditions {#Sec14}
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*Pseudomonas aeruginosa* strains 887 and 887-9G were cultured overnight in 3 % tryptone broth containing 0.3 % yeast extract (Difco, Detroit, MI, USA) (T3Y). Cultures were adjusted to an OD~600~ of 1.0 (10^8^ CFU/ml) in T3Y, and were incubated for 1 h at 37 °C with shaking at 180 rpm. Cells were then pelleted by centrifugation (5 min at 3000×*g*) and re-suspended in T3Y or different media: Brain heart infusion media
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