TRICALC 7.5
TRICALC 7.5
TRICALC 7.5
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And then I could not TRICALC 7.5 64 verschlusseln bilder modify the file to use the version I want.Oxygen software.. Overall, the frequency of these conditions was very similar to the reported frequencies of BCS in human astrocytes^[@CR18],[@CR58]^.
In the present study, the typical morphologies of astrocytes observed in human spinal cord include small cells with fine processes, large polygonal cells with prominent processes, flat cells with a star-like morphology, and stellate cells with a dense process growth. Astrocytes in the grey matter of the spinal cord are larger than those in the white matter^[@CR47]^. After culture, we observed a variety of astrocyte morphologies, including stellate, flattened, and polygonal cells, similar to the predominant shapes identified in human specimens. We also observed a few large astrocytes with notable processes in cultures. These morphological changes underlie changes in the expression of astrocytic markers, such as GFAP, Aqp4, and S100β, as well as mRNA expression of different types of ion channel genes in cultured astrocytes. We found that cultured astrocytes expressed the commonly used markers of human astrocytes. The increase in astrocytes’ S100β expression may reflect a change in the activation status of astrocytes following the acute injury of isolated spinal cords. S100β is a calcium-binding protein in the EF-hand-type family and is mainly present in Schwann cells and astrocytes^[@CR59]^. S100β is constitutively expressed by astrocytes, mainly at the level of P6 mice and increases with age^[@CR60]^. In our study, the expression of S100β increased, at least within 48 h in culture and peaked at 14 and 21 dpi. Thus, it may be a suitable marker of cultured astrocytes. Interestingly, we found that astrocytes without apparent morphological differences with respect to the in vivo or in vitro condition expressed GFAP. Together, these results suggest that cultured astrocytes from the white and grey matter of the spinal cord exhibit diverse morphological and
https://colab.research.google.com/drive/1wRonsOL5A8ldTY9poyn86s-Zaa2pPioo
https://colab.research.google.com/drive/17hgQdZnAtLqr117flRwdwry6aXZFuNIF
https://colab.research.google.com/drive/1vjCDbUvDR-1nvz3p-TrQReO5qlRa6kJ4
https://colab.research.google.com/drive/1IAp9IyB4Ylug-8BXe-Jc5ib6sDuwfpIj
https://colab.research.google.com/drive/1CwbICaKrM1E0L9Pk0tWE1Hdbb6IipCTG
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