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Rcd 2000 CD – Rockit Audio – 5 min read, 2 RNS 300 Navigation .New film-based colorimetric cell biosensor for detection of deoxyribonucleic acid.
We have developed a novel, easy-to-use, low-cost biosensor based on the principle of label-free colorimetric readout to detect the duplex structure of DNA in a homogeneous solution. The developed aptamer biorecognition element for in vitro screening of target molecules (thrombin) consists of a one-step reaction between 2′-hydroxyl groups in the DNA duplex and a target molecule. A pair of thiol-modified oligonucleotide probes (TPH) with a dithiobis(succinimidyl propionate) linker and an aminopropyl-modified duplex DNA (APM) with 6-carboxyfluorescein at each end were reacted in the presence of target molecules to form a fluorescent complex. The addition of the alkaline phosphatase-conjugated chitosan as a signal amplifier resulted in a significant color change from green to bright yellow. The detection limit is 2.0 ng/mL of thrombin. The relative standard deviation (RSD) for the determination of 5.0 and 100 ng/mL of thrombin was 5.6% (n = 5). The assay was selective and of high sensitivity. The proposed biosensor was successfully applied to the selective detection of thrombin in serum samples..

A:

This has been solved, it was an error in the way the loading was done in the MainActivity.
By changing the line:
public static void main(String[] args)

To
public static void main(String[] args) throws Exception

The problem was solved.

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