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Download Beauty And The Beast 480p | HDSUB | HD720p | WEB-DL (Beauty And The Beast Online 480p 720p Watch Online Free English..A rapid HPLC assay for thymidine kinase production in yeast.
A rapid HPLC assay for the assessment of thymidine kinase (TK) activity is described. The assay utilizes the production of 5-phosphorylated deoxynucleoside monophosphates by TK and the following separation of the phosphorylated deoxynucleoside monophosphates by reverse-phase HPLC. The generation of HPLC peaks of the phosphorylated deoxynucleoside monophosphates was analyzed by measuring the quantity of peak area. HPLC was compared with an existing HPLC method (Padayatty et al., Biotechnol. Bioeng. 32, 255-267, 1990) using recombinant TK in the presence of 5-phosphorylated deoxynucleoside monophosphates at various concentrations. A nonlinear regression analysis was used to compare the peak area data. This technique produced a linear relation between the amount of sample injected into the HPLC system and the peak area. The new technique was able to analyze samples as little as 20 microl. Data analysis was automated using a pocket calculator. The new assay was compared to a commercial test, Thymidine Kinase Assay, a radioisotopic assay. The results indicated that the HPLC technique for the measurement of TK activity was equivalent to the radioisotopic assay.Q:

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