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Anopheles funestus is the most important malaria vector in Western and central Africa. It is also becoming the main vector of malaria in the East African region. Adult An. funestus are blood feeders and the ability to adapt to such a complex feeding strategy is believed to be linked to the expression and activity of serine proteases involved in the digestive tract during blood meal acquisition. In this study, we obtained two full-length cDNA sequences (ag4RNAN and ag4RNAN2) encoding one unique serine protease-like protein containing a signal peptide at the N-terminal region. They contain two domain architectures: a papain-like cysteine protease catalytic domain and a Kazal-type peptide inhibitor domain. A phylogenetic tree based on the deduced amino acid sequences of the ag4RNAN and ag4RNAN2, similar to the other two model Anopheles species (An. gambiae and An. quadriannulatus), revealed that they are more closely related to An. gambiae (agap) than An. quadriannulatus (alpr) serine proteases. A low level of expression of these two genes has been observed in salivary glands of the eight-day-old adult females. In contrast, a very high expression was noted in the whole body. Further immunolocalisation analyses showed that the two ag4RNAN genes were highly expressed in the midgut in all developmental stages. The ag4RNAN2 gene was not expressed in the developmental stages tested; this result differs from the expression of the agap gene which was highly expressed in the midgut of all life stages. According to these results, we suggest that the presence of the agap protease gene might be a prerequisite for the expression of the two ag4RNAN genes in the midgut.

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Physicians who practice in the internal medicine subspecialty can be considered to be one of the most evidence-based physicians. In an effort to enhance their evidence-based practice, these physicians attended a workshop that provided them with training in the use of evidence and practice-based guidelines. During their routine practice, they were instructed to discuss evidence-based practice with their staff, to communicate with colleagues about changes that they had made in their practice, and to assess their practice’s quality of care. The aim of the present study was to evaluate the impact of the workshop on practice change. Physicians were mailed an anonymous questionnaire 1 and 2 years after the workshop to assess changes in their practice. Completed questionnaires were returned by 116 of the 147 physicians (79%). Physicians self-reported the following changes in practice: increased use of practice-based guidelines; increased use of evidence-based practice; greater focus on chronic illness care; decreased use of recommendations, which included: screening tests, treatments, and practices; and an increased focus on educating their patients. Participants also increased their practice-based guideline searching (p=0.001), the number of quality audits performed (p=0.0001), and the number of practice changes made (p=0.003). Physicians who responded to the questionnaire reported a significant increase in their practice change (p