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Substance P (SP) causes stimulation of cell proliferation and inhibition of apoptosis in leukemic cells. Anti-leukemia drugs are able to induce apoptosis. SP receptors (SP-R) have been detected in a wide variety of cell types and tissues. SP-R may regulate the sensitivity of the target cell to antineoplastic drugs. In this work, SP-R in human leukemia cell lines, K562, and HL60, were studied with the use of RT-PCR. The mRNA of SP-R was detected in both leukemia cell lines. A single-band PCR product of the size expected for SP-R was observed in each leukemia cell line. K562 or HL60 cells were cultured in the presence or absence of SP-R antisense oligonucleotide, and then SP or cytotoxic agents were added. SP caused proliferation of cells and inhibited cytotoxicity of chemotherapeutic agents in cell culture. The effects were reversed by SP-R antisense oligonucleotide. SP-R was detected in K562 and HL60 cells. The expression was suppressed by a SP-R antisense oligonucleotide. The suppression of SP-R expression increased the sensitivity of K562 cells to vincristine. The present results suggest that SP-R is expressed in leukemic cells and regulates sensitivity of the cells to various cytotoxic chemotherapeutic agents.In the final months of the Obama administration, the Department of Health and Human Services moved to make the first federal rules to implement ObamaCare.
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